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nuclei isolation buffer  (TaKaRa)


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    Structured Review

    TaKaRa nuclei isolation buffer
    Nuclei Isolation Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1914 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclei+isolation+buffer/Recombinant+RNase+Inhibitor/pm41723114-197-10-40
    Average 97 stars, based on 1914 article reviews
    nuclei isolation buffer - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Single-cell multiomic human brain atlas reveals regulatory drivers of cortical regionality.
    Article Snippet: .. Frozen tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 mM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl2, 3 mM MgAc2, 0.1% Triton X-100, 160 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue. .. Frozen tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 mM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl2, 3 mM MgAc2, 0.1% Triton X-100, 160 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue.

    Article Title: RNA isoform diversity in human neurodegenerative diseases
    Article Snippet: .. Frozen 300 μm tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 μM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl2, 3 mM MgAc2, 0.1% Triton X-100, 80 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue. ..

    Article Title: Single-cell multiomic human brain atlas reveals regulatory drivers of cortical regionality
    Article Snippet: .. Frozen tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 mM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl 2 , 3 mM MgAc 2 , 0.1% Triton X-100, 160 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue. ..

    Article Title: RNA Isoform Diversity in Human Neurodegenerative Diseases
    Article Snippet: RNA was isolated from 20 μm sections using the RNeasy Mini kit (Qiagen #74106) and analyzed on an Agilent 4200 TapeStation using an Agilent High Sensitivity RNA ScreenTape assay. .. Frozen 300 μm tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 μM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl 2 , 3 mM MgAc 2 , 0.1% Triton X-100, 80 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue. ..

    Isolation:

    Article Title: Single-cell multiomic human brain atlas reveals regulatory drivers of cortical regionality.
    Article Snippet: .. Frozen tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 mM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl2, 3 mM MgAc2, 0.1% Triton X-100, 160 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue. .. Frozen tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 mM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl2, 3 mM MgAc2, 0.1% Triton X-100, 160 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue.

    Article Title: RNA isoform diversity in human neurodegenerative diseases
    Article Snippet: .. Frozen 300 μm tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 μM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl2, 3 mM MgAc2, 0.1% Triton X-100, 80 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue. ..

    Article Title: Single-cell multiomic human brain atlas reveals regulatory drivers of cortical regionality
    Article Snippet: .. Frozen tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 mM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl 2 , 3 mM MgAc 2 , 0.1% Triton X-100, 160 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue. ..

    Article Title: RNA Isoform Diversity in Human Neurodegenerative Diseases
    Article Snippet: RNA was isolated from 20 μm sections using the RNeasy Mini kit (Qiagen #74106) and analyzed on an Agilent 4200 TapeStation using an Agilent High Sensitivity RNA ScreenTape assay. .. Frozen 300 μm tissue sections were incubated in 1 ml of cold nuclei isolation buffer [320 mM sucrose, 100 μM EDTA, 1 μM DTT, 10 mM Tris-HCl pH 8, 5 mM CaCl 2 , 3 mM MgAc 2 , 0.1% Triton X-100, 80 U/ml RNase inhibitor (Takara Bio #2313A), cOmplete EDTA-free protease inhibitors (Roche #11836170001)] for 15 min and homogenized using a tissue grinder (Wheaton #358005) 10–15 times to mechanically dissociate the tissue. ..



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